Somaye Tayefeh; Naser Mahna; Seyed kamal Kazemitabar; Vali Ghasemi-Omran
Volume 3, Issue 2 , March 2018, , Pages 29-39
Abstract
Hyssops are herbaceous and perennial plants, belonging to the Lamiaceae family. In this experiment, callus production of two hyssop species (H. officinalis and H. angustifolius called ...
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Hyssops are herbaceous and perennial plants, belonging to the Lamiaceae family. In this experiment, callus production of two hyssop species (H. officinalis and H. angustifolius called common and local, respectively) was investigated using two culture media and different levels of plant growth regulators (PGR’s) to achieve the best culture medium and PGR combination for producing callus as a starting plant material for plant regeneration and secondary metabolite production. To do this, the seeds were cultured in MS medium and after 2 weeks shoots were placed on to the MS and B5 medium and PGR treatments (control, 0.5mg/L 2,4-D, 1mg/L 2,4-D, 0.2 mg/L NAA + 0.5mg/L 2,4-D, 0.2 mg/L NAA + 1mg/L 2,4-D) and incubated in a controlled environment. Then, the callus induction time and percentage of callus producing explants, fresh and dry weight of callus and callus color were recorded. The results showed that interaction between species, medium and PGR treatments was significant at 1% level. Meanwhile, mean comparison analyses showed that the highest amount of callus production in both evaluated hyssop species was observed in MS media supplemented with 0.5 mg/L 2,4-D and 0.2 mg/L NAA, in which the common hyssop showed calli after 6 days, while the local hyssop species started callusing after 11 days. According to this research, this PGR treatment is proposed for callus induction in both species. Given the importance of these medicinal speciesof hyssop especially the local one and probability of its extinction, these results can be used for protecting this valuable herb.
